Overall differences between the RVD-only and the RVD + HDM arm. (A) Phase 3 study design and clinical features of patients in the IFM 2009 study. Overall comparisons between the 2 arms in this study, and selected samples analyzed in this study are shown in the table. (B) Number of mutations for samples in RVD-alone arm and RVD + HDM arm at diagnosis (left) and relapse (right). y-axis shows number of mutations in each group and x-axis shows groups. P values are shown (top). (C) For patients, (x-axis) all detected mutations at diagnosis and relapse are taken, and the percentage of contribution from 3 categories (light blue: mutations only detected at diagnosis; dark blue: mutations detected at both time points (shared), 6; and green: mutations only detected at relapse) are shown (y-axis). Patients treated with RVD alone (left) and patients treated with RVD + HDM (right) are shown. (D) Number of SVs (y-axis) for deletions, duplications, inversions, and translocations (left to right) at diagnosis (orange) and relapse (light blue) (x-axis) for patients treated with RVD + HDM. (E-F) Copy number profiles for patients treated with RVD + HDM at diagnosis (E) and relapse (F). Left side of each panel shows the number of patients (% is given on right) with gain (red) and deletions (blue) from chromosome 1 to X (x-axis). Light and dark blue and red colors are separating p and q arms of each chromosome. IQR, interquartile range; max, maximum; min, minimum; PR, partial response; R, lenalidomide; sCR, stringent complete response; VGPR, very good partial response.