RNA-seq analysis determines key pathways that are altered by low levels of Myb. (A) UpSet plot showing the significantly (adjusted P < .10) DE genes from RNA-seq comparisons of WT and Myb^+/− KSL from young (2-month) vs older (12-month) animals. Each row represents the different conditions where points to the right of the sets provide information about the intersecting sets of the different conditions. The vertical bars indicate the number of intersecting DE genes in each combination of conditions. The set size below the horizontal bars represents the number of DE genes within each set (condition). The UpSet plot was generated using Intervene (version 0.6.4). (B) KEGG pathway analysis from the comparison of 2-month-WT vs Myb^+/− and 12-month-old WT and Myb^+/− KSL cells. The top 10 most significant pathways are depicted based on their FDR. (C) The percentage of genes in each depicted pathway that are significantly DE in 2- and 12-month-old KSL. (D) Quantitative PCR of RNA expression in aged Myb^+/+:CreER^T2:mTmG and Myb^+/Δ:CreER^T2:mTmG sorted GFP+ KSL cells. Values represent the mean expression plotted as 2^−ΔΔCT with SEM n = 3. DE, differentially expressed; FDR, false discovery rate; SEM, standard error of the mean.