Longitudinal single-cell RNA-seq analysis of MCL during OAsIs. (A) UMAP plots of the cells from patient MCL34 at inclusion (incl.) and at relapse (Rel) after OAsIs treatment. Each dot represents a single cell. UMAP plots display the NIK/ NFκB2 (left panel), NFκB1 (middle panel) and BCR (right panel) signature scores at incl. and Rel. Wilcoxon sign-rank test; ∗∗∗∗P_adj < .0001. (B) UMAP plot of the 7 clusters identified by Seurat at incl. and at Rel after OAsIs treatment for the patient MCL34. The cell proportion for each cluster at incl. (green) or Rel (pink) is indicated at the bottom. (C) UMAP plot displaying the cell-cycle signature scores at incl. and Rel for the patient MCL34. Cell proportion of cycling (red) or resting (gray) in each cluster is indicated at the bottom. (D) Trajectory of single cells at incl. and at Rel using Monocle2. Clusters defined in panel B are identified in the lower panel. (E) Heatmap showing the top 20 genes for each cluster. The color gradient indicates the intensity of the median gene expression as indicated. Functional enrichment pathway was calculated using Reactome and Enrichr. ∗∗∗∗P_adj < .0001. UMAP, Uniform Manifold Approximation and Projection.