Figure 6.
CD53 supports p107 and p130 protein expression. (A) Representative maximum projection confocal images of total p107 (red) and DAPI (blue) in individual HSCs with a single XY differential interference contrast (DIC) image. (B) Quantification of the total p107 intensity in the RFU from HSCs from the indicated groups (n = 30-50 cells per group) over 3 independent experiments. Average RFU per mouse from at least 20 individual HSCs per sample is shown. The average spread in a single mouse can be seen in supplemental Figure 5A. (C) Representative confocal images of Thr369 phospho-p107 (red) and DAPI (blue) in individual HSCs. (D) Quantification of Thr369 p-p107 intensity in HSCs from the indicated groups (n = 29-42 cells per group) in 3 independent experiments. The average RFU per mouse from at least 20 individual HSCs per sample is shown. The average spread in a single mouse can be seen in supplemental Figure 5B. (E) The ratio of p-p107 to total p107. (F) Representative confocal images of total p130 (red) and DAPI (blue) in individual HSCs. (G) Quantification of total p130 in RFU from HSCs from the indicated groups (n = 37-66 cells per group) in 3 independent experiments. The average RFU per mouse from at least 20 individual HSCs per sample is shown. The average spread in a single mouse can be seen in supplemental Figure 5C. (H) Representative confocal images of Ser639 p-p130 (red) and DAPI (blue) in individual HSCs. (I) Quantification of Ser639 p-p130 intensity in HSCs from the indicated groups (n = 35-41 cells per group) over 3 independent experiments. The average RFU per mouse from at least 20 individual HSCs per sample is shown. The average spread in a single mouse can be seen in supplemental Figure 5D. (J) The ratio of p-p130 to total p130. All fluorescent images are maximum intensity projections, whereas the DIC images are of a single XY plane. All scale bars are 5 μm. Error bars represent the mean ± standard deviation. ∗P < .05; and ∗∗P < .01 by an unpaired Student t test. DAPI, 4',6-diamidino-2-phenylindole.

CD53 supports p107 and p130 protein expression. (A) Representative maximum projection confocal images of total p107 (red) and DAPI (blue) in individual HSCs with a single XY differential interference contrast (DIC) image. (B) Quantification of the total p107 intensity in the RFU from HSCs from the indicated groups (n = 30-50 cells per group) over 3 independent experiments. Average RFU per mouse from at least 20 individual HSCs per sample is shown. The average spread in a single mouse can be seen in supplemental Figure 5A. (C) Representative confocal images of Thr369 phospho-p107 (red) and DAPI (blue) in individual HSCs. (D) Quantification of Thr369 p-p107 intensity in HSCs from the indicated groups (n = 29-42 cells per group) in 3 independent experiments. The average RFU per mouse from at least 20 individual HSCs per sample is shown. The average spread in a single mouse can be seen in supplemental Figure 5B. (E) The ratio of p-p107 to total p107. (F) Representative confocal images of total p130 (red) and DAPI (blue) in individual HSCs. (G) Quantification of total p130 in RFU from HSCs from the indicated groups (n = 37-66 cells per group) in 3 independent experiments. The average RFU per mouse from at least 20 individual HSCs per sample is shown. The average spread in a single mouse can be seen in supplemental Figure 5C. (H) Representative confocal images of Ser639 p-p130 (red) and DAPI (blue) in individual HSCs. (I) Quantification of Ser639 p-p130 intensity in HSCs from the indicated groups (n = 35-41 cells per group) over 3 independent experiments. The average RFU per mouse from at least 20 individual HSCs per sample is shown. The average spread in a single mouse can be seen in supplemental Figure 5D. (J) The ratio of p-p130 to total p130. All fluorescent images are maximum intensity projections, whereas the DIC images are of a single XY plane. All scale bars are 5 μm. Error bars represent the mean ± standard deviation. ∗P < .05; and ∗∗P < .01 by an unpaired Student t test. DAPI, 4',6-diamidino-2-phenylindole.

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