BCAR1 is a new BTG1 interactant. (A) Volcano plot of the proteins identified after anti-HA immunoprecipitation in cells transfected with HA-BTG1 or HA-GFP plasmids (n = 3). (B) Coimmunoprecipitation confirmation of the BTG1-BCAR1 interaction (representative of n = 3 experiments). (C) Illustrative confocal acquisitions (original magnification ×40) (left) and quantification (right) measuring mCherry fluorescence (transfection control), DAPI (nuclear staining), and Venus fluorescence (BiFC) after transfection with VN-BTG1 and CC-MDH2 (negative control) or CC-BCAR1, as indicated (representative of 4 experiments).