Effect of BTG1 deletion on actin cytoskeleton, cell adhesion, and migration. (A) Representative image and (B) quantification of phalloidin staining of TMD8-BTG1^WT and TMD8-BTG1^−/− cells on poly-L-lysine–coated slides (n = 10 for each group). (C) Confocal images in vertical (α = 0°) and horizontal (α = 90°) planes of phalloidin (purple) and Hoechst (blue) staining of TMD8-BTG1^WT, TMD8-BTG1^−/−, and TMD8-BTG1^rescue on fibronectin-coated slides, with (D) quantification of TMD8-BTG1^WT, TMD8-BTG1^−/−, TMD8-BTG1^rescue, and TMD8-BTG1^{rescuemutated} cell area (P < .0001, compared with TMD8-BTG1^WT, n = 3). (E) Cell index measurement (Xcelligence) after addition of TMD8-BTG1^−/− (blue), TMD8-BTG1^WT (red), or TMD8-BTG1^rescue (purple) on fibronectin-coated or uncoated wells. (F) Quantification of cellular motility of TMD8-BTG1^WT, TMD8-BTG1^−/−, TMD8-BTG1^rescue, and TMD8-BTG1^{rescuemutated} seeded onto fibronectin-coated slides (P < .0001, compared with TMD8-BTG1^WT, n = 3). Values represent mean ± SEM; ∗∗∗∗P < .0001, using one-way ANOVA with Dunnett multiple comparisons test in panels D,F. ANOVA, analysis of variance; ns, not significant; SEM, standard error of the mean.