Upregulation of OX40 on activated T cells. (A) Nonhuman primate (NHP) model setup for panels B-C. (B-C) Percentage of OX40⁺ CD4⁺ or CD8⁺ T cells detected in (B) peripheral blood (Auto: n = 6; GVHD: n = 10) at specified time points or (C) tissues upon terminal analysis (Auto: n = 4; GVHD: n = 6). Mean ± standard error of the mean values are shown. In panel C, each dot represents data from an individual animal. P values were calculated using 2-way analysis of variance (ANOVA) followed with Holm-Sidak correction. (D) Percentage of OX40⁺ primary human T cells within total CD4⁺ or CD8⁺ T cell population at specified time points after OKT3/αCD28 stimulation (n = 6). Mean ± standard deviation (SD) values are shown. (E) MLR model setup for panel F. (F) Percentage of OX40⁺ cells within the specified T cell population on day 7 after MLR setup. Each line connects data from the same pair of stimulator and responder. P values were calculated using paired t test. BAL, bronchoalveolar lavage; mLN, mesenteric lymph node; pLN, popliteal lymph node; TBI, total body irradiation.