Fast- and slow-dissociating AIM-A1 molecules describe a 2-state binding model to GPIbα. (A) Representative sensorgrams of indicated AIM-A1 variants at concentrations from 1 μM to 15.625 nM show that type 2B VWD mutants yield greater binding response to immobilized PT-LBD (W230L). (B) Steady-state binding data of sensorgrams displaying means ± standard deviations of n = 3 independent replicates.