Figure 4.
The fission of mitochondria during platelet activation/spreading does not depend on microtubules or acto-myosin contraction. (A) Comparison of the number of mitochondria in platelets spread for 60 minutes on a glass surface under different assay conditions. Resting platelets were stained with mitoTracker and then incubated for 30 minutes either at RT (intact microtubules) or at 4°C (depolymerized microtubules). Platelets were then centrifuged onto glass surface in the cold before being placed at 37°C for 60 minutes. The graph illustrates the number of mitochondria in platelets combined from 4 different donors. Data comparison was performed using the 2-tailed Mann-Whitney test. (B-C) Comparison of the number of mitochondria in platelets spread for 60 minutes on a glass surface under different assay conditions. Resting platelets were stained with mitoTracker and then incubated for 30 minutes at RT with vehicle (C), 10 μM y-27632 (Y) or 50 μM blebbistatin (B). Platelets were then centrifuged onto the glass surface and placed at 37°C for 60 minutes. The graph illustrates the number of mitochondria in platelets combined from 2 different donors. Data comparison was performed using the 2-tailed Mann-Whitney test.

The fission of mitochondria during platelet activation/spreading does not depend on microtubules or acto-myosin contraction. (A) Comparison of the number of mitochondria in platelets spread for 60 minutes on a glass surface under different assay conditions. Resting platelets were stained with mitoTracker and then incubated for 30 minutes either at RT (intact microtubules) or at 4°C (depolymerized microtubules). Platelets were then centrifuged onto glass surface in the cold before being placed at 37°C for 60 minutes. The graph illustrates the number of mitochondria in platelets combined from 4 different donors. Data comparison was performed using the 2-tailed Mann-Whitney test. (B-C) Comparison of the number of mitochondria in platelets spread for 60 minutes on a glass surface under different assay conditions. Resting platelets were stained with mitoTracker and then incubated for 30 minutes at RT with vehicle (C), 10 μM y-27632 (Y) or 50 μM blebbistatin (B). Platelets were then centrifuged onto the glass surface and placed at 37°C for 60 minutes. The graph illustrates the number of mitochondria in platelets combined from 2 different donors. Data comparison was performed using the 2-tailed Mann-Whitney test.

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