Molecular monitoring of germ line RUNX1 carriers with CH. (A) Driver somatic variants identified in RUNX1 carriers-without HM individuals across age. Circle size = increasing VAF, color = gene. Individuals with unknown age were given the value 0 (D01_2, D02_2, S_E_4, U02_3, U02_4). (B) Longitudinal case studies with the VAF of detected driver somatic variants plotted across years. Clinical diagnosis at the time of monitoring is indicated below the age of the individual at which the sample was collected. AML, acute myeloid leukemia; TCP, thrombocytopenia.