Figure 2.
Loss of PIK3R1 leads to resistance to Notch inhibition in T-ALL cells. (A) Cell proliferation assays of T-ALL PIK3R1 KO cell lines under DMSO, CB-103, or GSI treatment conditions. Gray connected dots, NT control; and colored dots, representative PIK3R1 KO cell lines. (B) Cell cycle analyses of PIK3R1 KO cell lines performed 6 days after DMSO or GSI treatment at indicated concentrations. (C) Cell cycle analyses of PIK3R1 KO cell lines 24 hours after DMSO or CB-103 treatment at indicated concentrations. (D) Apoptosis assays of PIK3R1 KO cell lines performed 3 days after DMSO or CB-103 treatment at indicated concentrations. Experiments shown here were performed with 2 independent T-ALL cell lines, DND-41 and RPMI-8402. The values shown are mean ± standard deviation (SD) (n = 3 biologically independent samples, 2 independent experiments). One-way analysis of variance (ANOVA); nonsignificant (ns); ∗P < .0332; ∗∗P < .0021; ∗∗∗P < .0002; ∗∗∗∗P < .0001.

Loss of PIK3R1 leads to resistance to Notch inhibition in T-ALL cells. (A) Cell proliferation assays of T-ALL PIK3R1 KO cell lines under DMSO, CB-103, or GSI treatment conditions. Gray connected dots, NT control; and colored dots, representative PIK3R1 KO cell lines. (B) Cell cycle analyses of PIK3R1 KO cell lines performed 6 days after DMSO or GSI treatment at indicated concentrations. (C) Cell cycle analyses of PIK3R1 KO cell lines 24 hours after DMSO or CB-103 treatment at indicated concentrations. (D) Apoptosis assays of PIK3R1 KO cell lines performed 3 days after DMSO or CB-103 treatment at indicated concentrations. Experiments shown here were performed with 2 independent T-ALL cell lines, DND-41 and RPMI-8402. The values shown are mean ± standard deviation (SD) (n = 3 biologically independent samples, 2 independent experiments). One-way analysis of variance (ANOVA); nonsignificant (ns); ∗P < .0332; ∗∗P < .0021; ∗∗∗P < .0002; ∗∗∗∗P < .0001.

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