Hepcidin expression is not inducible by iron in double Bmp5^se-Bmp6^fl-Tek-Cre⁺ mutant mice. Three-week-old male (open circles) and female (closed circles) double Bmp5^se-Bmp6^fl-Tek-Cre⁺ mutant mice and littermate single Bmp5-mutant mice (Bmp5^se-Bmp6^fl-Tek-Cre-) were maintained on a low-iron diet (2-6 ppm iron) at weaning for 3 weeks to prevent iron overload induced by a standard rodent diet. Mice were either maintained on a low-iron diet (L) or switched to a matched purified adequate-iron diet (L-A; 48 ppm iron) for 1 more week. Mice were analyzed for liver Hamp (A) and Id1 relative to Rpl19 mRNA (B) via qRT-PCR. (C) Liver iron was analyzed using a colorimetric assay. Liver Bmp6 (D) and Bmp2 relative to Rpl19 mRNA (E) were analyzed via qRT-PCR. For panels A-B and D-E, the average of single Bmp5 mutant male mice on the low-iron diet was set to 1. For all graphs, individual data points are shown, and bars represent mean ± SEM. Sex-matched data were analyzed using 2-way ANOVA with Tukey post hoc test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 for the indicated comparisons.