BMP5 contributes to hepcidin regulation in 10-day-old mice. (A-C) Ten-day-old and 8-week-old male (open circles) and female (closed circles) Bmp5^wt, Bmp5^het, and Bmp5^se mice were analyzed for liver Hamp relative to Rpl19 mRNA via quantitative polymerase chain reaction (qRT-PCR) (A). The average of the 10-day-old wild-type male mice was set to 1. Serum transferrin (Tf) saturation (B) and liver iron levels (C) were measured using colorimetric assays. (D) Copy number of liver Bmp5 and Bmp6 mRNA was quantified via qRT-PCR in wild-type male (open symbols) and female (closed symbols) mice at ages 10 days (triangles) and 8 weeks (squares). For all graphs, individual data points are shown, and bars represent the mean ± standard error of the mean (SEM). For panels A-C, age- and sex-matched data were analyzed using 1-way analysis of variance (ANOVA) with Tukey post hoc test. For panel D, sex-matched data were analyzed using 2-way ANOVA with Tukey post hoc test. ∗P < .05; ∗∗ P < .01; ∗∗∗P < .001 for the indicated comparisons.