NGS-based T-cell clonality analysis identifies clonal TR gene rearrangements in cHL samples. (A) Representative data set indicating NGS-based detection of TR gene rearrangements in paired diagnosis (D) and recurrence (R) tissue samples of a case with a dominant T-cell clone in only the primary diagnosis but not recurrence (case 34). The results of 2 TCR targets (TRBV-TRBD-TRBJ and TRGV-TRGJ) are shown, and the specific clonotypes for the dominant TR gene rearrangements are indicated. On the x-axis, the junction aa length is shown, and the abundancy of clonotypes is shown in percentages on the y-axis. (B) Representative data set indicating NGS-based detection of TR gene rearrangements in paired samples with a preserved identical dominant T-cell clone (case 1). (C) Summary of IG- and TR-NGS clonality results of all patients with at least 1 sample with a dominant TCR clone. EBV status of all tumor samples and age of the patients are shown.