NliG-motif drives FL cells from light to dark zone type GC B cells. (A) Most variable genes between NliG motif–positive and NliG motif–negative cells (58 genes) were used for unsupervised clustering. The top row depicts the Seurat cluster for each cell, and second row shows BCR NliG motif status. Vertical annotation (primary y-axis) depicts DZ (orange) and LZ (yellow) genes in healthy GC B cells (gene names in bold on secondary y-axis). (B-C). Average gene expression in the pseudobulk of NliG motif–positive and NliG motif–negative single cells. Genes were analyzed that were significantly differentially expressed in single cell data and were characteristic for DZ (B) and LZ (C) GC B cells. Sets of genes were obtained from data published by Victora et al⁵ (vict), Milpied et al⁶ (mil), Michaelsen et al¹⁵ (mich), or Tripodo et al¹⁶ (trip). pos, positive; neg, negative.