Expression of BCR with NliG-motif affects gene expression and subclonal diversity. (A) Partial heavy chain sequences of germ line, NliG-motif–negative subclone 1, and NliG motif–positive subclone 2. Nucleotide and amino acid substitutions in subclones 1 and 2 compared with those in the germ line are highlighted. The NliG motif is annotated in black. (B) Uniform manifold approximation and projection (UMAP) dimensionality reduction and clustering of single FL cells based on the transcriptome profiles. (C) Distribution of NliG motif–negative cells and NliG motif–positive cells per gene expression cluster. (D) Shannon-Weaver indices of diversity were calculated and compared according to Hutchenson's method and corrected for multiple testing. (E) BCR subclonal composition per gene expression cluster. Shannon-Weaver index of diversity (H) is included in the graph titles. Pie colors are randomly picked and are not associated with gene expression clusters. (F) Mean AICDA gene expression between gene expression clusters. pos, positive; neg, negative; ns, not significant.