Effects of HDL and LDL on VWF self-association in microfluidic devices. (A-D) Solutions containing purified VWF (7.5 μg/mL) in phosphate-buffered saline, fluorescein isothiocyanate (FITC)–conjugated anti-VWF antibody, and HDL (2.4 mg/mL) or LDL (2.2 mg/mL) were perfused through microfluidic devices at an upstream wall-shear rate of 9300 s−1. VWF fibers formed around the micropost were monitored for both DIC and FITC intensities. Data were from 3 to 6 experiments. (A) DIC images of VWF fibers formed after 8 minutes of perfusion. (B) VWF fibers, proportional to the mean fluorescence intensity of the bound FITC-conjugated anti-VWF antibody, were quantified over time. Shading represents standard deviations (SDs). The length and volume of VWF fibers at the end of perfusion were quantified in (C) and (D), respectively. (E-H) Citrated plasma from healthy donors, with phosphate-buffered saline, HDL (1 mg/mL), or LDL (1 mg/mL) were perfused through microfluidic devices. VWF fibers formed around the micropost were monitored using DIC intensity. Data were from 3 to 7 experiments. (E) DIC images of VWF fiber formation after 8 minutes of perfusion. (F) VWF fiber areas were quantified over time. Shading represents SDs. (G) The lengths of VWF fibers at the end of perfusion were quantified. (H) The initial rates of VWF fiber formation were calculated. ∗P < .05; ∗∗P < .01; and ∗∗∗P < .001 by t test.
