Figure 6.
Effect of HRG on catheter-induced clotting in control or HRG-depleted human plasma. Control human plasma (A), or HRG-depleted human plasma (B) was incubated with human HRG up to 5 μM in the absence (red line) or presence (blue line) of catheter segments for 15 minutes at 37°C before initiating clotting via the addition of 20 mM CaCl2. Absorbance was monitored and clot time was determined as the half-time of maximal absorbance. Mean ± SD of 4 determinations. ∗P < .05; ∗∗P < .01; and ∗∗∗P < .001 comparisons of the clot time in the absence of catheter segments and without HRG; #P < .05 and ##P < .01 compared with clot time in the presence of catheter segments and without HRG (one-way ANOVA and Holm-Sidak method).
