RNA expression in platelets and whole blood of patients with MPN and protein expression in platelets. (A) MPN platelet RNA-seq normalized expression data: patients with MPN (total, n = 99; ET, n = 24; PV, n = 33; and MF, n = 42) vs healthy donors (n = 21) annotated further by (i) shape for JAK2 mutation status (triangle = yes and open circle = no), (ii) filled circle size for CALR mutation status, and (iii) color for variant allele fraction (VAF) for each patient. (B) Whole blood RNA: patients with MPN (total, n = 109; ET, n = 39; PV, n = 38; and MF, n = 32) vs healthy donors (n = 10). (C) Platelet protein: peripheral blood platelets were isolated from patients with MPN (total, n = 38; MF, n = 12; PV, n = 19; and ET, n = 7) and healthy donors (n = 11). Bonferroni multiple comparisons adjusted P values, ∗∗∗∗P < .0001; ∗∗∗P < .001; and ∗∗P < .01 when compared with controls (healthy donors). All RNA expression is normalized to glyceraldehyde-3-phosphate dehydrogenase and expressed as a log2 fold change.