Activating FcγRs regulate anti-Duffy–mediated HOD RBC removal by multiple immune populations in the spleen. (A) Schematic of experiment shown. CFSE-labeled HOD RBCs were transfused into B6 or FcγR KO mice in the presence or absence of anti-Duffy antibodies. (B) A partial summary of the flow-cytometry gating strategy used to detect CFSE⁺ immune populations. (C-E) Examination of distinct immune populations for CFSE positivity after exposure of B6 or FcγR KO mice to CFSE-labeled HOD RBCs in the presence or absence of anti-Duffy antibodies, including neutrophils (PMNs) (C), red pulp macrophages (RPMs; D), and DCs (E); n = 3 to 4 mice per group. Quantitative analysis of CFSE⁺ TER119⁻ events are shown. All plots show mean values ± standard deviation. ∗P < .05; ∗∗P < .01; and ∗∗∗P < .001. These results are representative of 3 independent experiments.