The dose of transfused HOD RBCs and the presence of anti-Duffy antibodies influences RBC alloimmunization. (A) Schematic representation of nonimmunized or anti-Duffy–antibody passively immunized recipient B6 mice exposed to 107, 108, or 109 HOD RBCs followed by examination of anti-HOD antibody formation. (B-G) Nonimmunized or anti-Duffy–antibody passively immunized recipient B6 mice were exposed to 107, 108, or 109 HOD RBCs, followed by detection of anti-HEL IgM on days 3 (B), 5 (C), and 7 (D), and of IgG on days 14 (E), 21 (F), and 28 (G) after transfusion; n = 10 mice per group. All plots show mean values ± standard deviation. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; and ∗∗∗∗P < .0001. These results are representative of 3 independent experiments.
