Single-cell atlas of NFIX-deficient BM hematopoietic progenitors. (A) Nfixflox/floxRosa26-CreERT2+/+ (Nfix+/+) and Nfixflox/floxRosa26-CreERT2+/T (NfixΔ/Δ) mice were treated with TAM for 14 days. Nfix+/+ and NfixΔ/Δ BM were collected and incubated with CITE-seq and FACS antibodies. Lineage−cKit+ BM cells, HSPCs, CMPs, and LT-HSCs were sorted and pooled 7:1:1:1 before processing for scRNA-seq and cell-surface protein abundance via 10x genomics. (B) Weighted nearest neighbor UMAP. Single cells from Nfix+/+ and NfixΔ/Δ BM clustered into 11 cell types based on cell-surface markers and RNA expression. (C) Protein abundance of 7 cell-surface markers representing the CITE-seq antibody signal (see also supplemental Figure 2). (D) Dot plot representing gene expression of specific cell marker genes across the 11 cell types. ERP, erythroid progenitors.
