The expression of YBX1 is elevated in T-ALL. (A) The mRNA levels of YBX1 were analyzed in healthy T cells and primary T-ALL cells using microarray data set GSE146901. (B) YBX1 mRNA levels in healthy bone marrow (BM) and T-ALL BMs from microarray data set GSE26713 were analyzed. (C) mRNA expression analysis of YBX1 in T-ALL compared with all cancer types and other hematologic cancers in the Cancer Cell Line Encyclopedia (CCLE) database. (D) The mRNA expression of YBX1 in T cells from healthy donors and patients with T-ALL was determined via qRT-PCR. (E) Relative YBX1 mRNA levels in T cells derived from healthy donors, Jurkat, Molt4, and CCRF-CEM cells. (F) YBX1 mRNA expression was detected in healthy T cells from wild-type mice and leukemia cells from NOTCH1-driven T-ALL mice. (G-I) Western blot analysis of YBX1 expression in healthy T cells and leukemia cells from patients with T-ALL (G), various patient-derived leukemia cell lines (H), and NOTCH1-induced T-ALL mice (I). Glyceraldehyde-3-phosphate dehydrogenase was used as an internal control. (J) Analysis of YBX1 expression in 264 primary T-ALL samples classified into 8 subtypes. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. N, normal T cells.