Evolution of TCR clonotypes at diagnosis and posttransplantation relapse in pediatric patients with AML. (A) Pie chart showing public TCR clonotypes found across >1 patient. (B) UMAP, TRB amino acid sequence, and cell counts for top 10 TCR clones based on size across all 4 patients. (C-D0 Pie charts showing number of each TCR clonotype identified at diagnosis (C) and at posttransplantation relapse (D) within a single patient, UPN4. (E) Alluvial description of T-cell phenotype probabilities within clonotypes from diagnosis to posttransplantation relapse for UPN4. (F) Phenotype of TCR clonotypes identified in diagnosis and/or posttransplantation relapse for UPN-4. ISG, IFN-stimulated gene.