DCA treatment of bone marrow progenitor cells increased the generation of CFU-GMs in the absence of stromal cells. (A) LSK cells were FAC-sorted from C57BL/6 marrow and placed into colony-forming media in the presence or absence of DCA. Cultures on day 11 were assayed for the presence of CFU-GMs, BFU-Es, and CFU-GEMMs and analyzed as (B) the proportion of total colonies present, (C) the total number of colonies, as well as the total number of (D) CFU-GMs, (E) BFU-Es, and (F) CFU-GEMMs. All data are shown and represent 3 experimental replicates and were analyzed using two-way ANOVA with Šidák post hoc test or an unpaired Student t test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.