The septin/ANK1 ratio correlates with SPT covering/distribution and RBC alteration score, and different septins differentially correlate with RBC morphology and functionality parameters. (A) Correlation between septins and ANK1 content presented in Figure 3G-J and Figure 2B. (B-D) Correlation between septin/ANK1 ratios calculated from Figure 3G-J and cytoskeletal parameters presented in Figure 2F-G or the RBC alteration score from Figure 1N. (E-K) Relation of the septin/ANK1 ratio with RBC morphology (E-F), surface-to-volume ratio (G), osmotic fragility (H), cryohemolysis (I), intracellular calcium content (J), and EV release (K). Data are respectively from Figure 1B-C,G, H-K. Correlations with septin-2, squares and blue linear regressions; with septin-7, circles and red linear regressions; with septin-8, triangles and green linear regressions; and with septin-11, inverted triangles and gray linear regressions. Linear regressions were plotted only for r² > 0.5; when 1 parameter correlates with 1 septin but not with the others, only the one correlating is indicated for the sake of clarity.