scRNA-seq confirms C3AR1 expression in NPM1-mutated AML blasts and in normal monocytes. (A) UMAP showing NPM1-mutated AMLs (n = 12), NBM MNCs (n = 5), and NBM CD34⁺ (n = 3) samples combined. (B) UMAP showing mutation calling of NPM1 in the same AML cells and projection as in panel A. Red: cells with detected NPM1 mutations. Gray: cells with WT NPM1 or no detected NPM1 transcript. (C) UMAP showing the expression of C3AR1 in same cells and projection as in panel A. Red indicates high expression. (D) UMAP showing the expression of GPR56 in same cells and projection as in panel A. Red indicates high expression. UMAP, uniform manifold approximation and projection for dimension reduction.