IQGAP1 interacts with CD11c. (A) Predicted interaction of top-hit proteins pulled down by immunoprecipitation (supplemental Figure 13) by STRING software. (B) Immunoprecipitation assay. Cell lysates of HL-60 cells were incubated with anti-human CD11c mAb (clone: CBR p150 2c1) for immunoprecipitation followed by blotting with anti-IQGAP-1 antibody or anti-CD11c polyclonal antibody. Cell lysates of 32D cl3 cells were incubated with anti-mouse CD11c mAb (clone: N417) for immunoprecipitation, followed by blotting with anti-IQGAP-1 antibody. What is shown is representative of 3 independent experiments. (C-E) CD11b (C), ROS (D), and phagocytosis (E) analyzed on HL-60 cells at 4 days under differentiation toward neutrophils. Three independent cell pools were analyzed in both WT and IQGAP1-KO groups. Shown are 1 of 3 independent experiments with the same pattern. (F) Apoptosis analysis by detecting cleaved caspase-3 at day 4 under differentiation toward neutrophils. HL-60 cells were treated with medium (control) or LPS (10 μg/mL) for 6 hours. Shown are 1 of 3 independent experiments with the same pattern. (G) Scheme of the role of CD11c-IQGAP1 interaction in neutrophil maturation. mAb, monoclonal antibody.