Constitutive activation of CD11c enhances neutrophil maturation and functions. (A) Scheme showing the strategy for creating the constitutively active CD11c KI mice (CD11cI334G). (B) Rosetting assay confirming the constitutive activation of CD11c molecule in CD11cI334G mice. Magnesium/calcium ions (1 mM) nonactivating condition, 1 mM manganese ions activating condition. (C) Giemsa staining of BM neutrophils. (D) Total BM neutrophil count. (E) Ratios of preneutrophils and immature and mature neutrophils. (F) Cell number and CXCR2 expression of blood neutrophils. (G) Cell number and CXCR2 expression of splenic neutrophils. (H) ROS detection in immature and mature neutrophils from naive WT and CD11c I334G mice. Left: representative flow cytometry overlay plot; right: data from 3 independent experiments. Cells from 3 to 4 mice were pooled together as 1 biological sample. (I) Neutrophil counts in the blood, the spleen, and the BM from WT and CD11cI334G mice, 6 hours after IV LPS stimulation. (J-K) WT and CD11c I334G mice were challenged intraperitoneally with E coli (1 × 108 colony-forming units) and sacrificed 6 hours later. (J) Giemsa staining of peritoneal neutrophils. (K) Bacterial loads of peritoneal cavity. ∗P < .05, ∗∗P < .01.
