CD11c deficiency leads to impaired neutrophil effector functions. (A) Akt phosphorylation in preneutrophils and immature and mature neutrophils in the BM of WT and CD11c KO mice. Data are mean ± SEM of 3 experiments. (B) ROS generation of sorted immature and mature neutrophils with or without PMA. BM cells from 3 mice were pooled as 1 biological sample. Upper panel: representative overlay analysis; bottom panel: mean ± SEM of mean fluorescence intensity (MFI) of 3 experiments. (C) Bulk RNA sequencing analysis comparing the transcriptome of sorted BM mature WT and CD11cKO neutrophils. Representative of 2 independent analyses with a similar pattern. (D-E) CD11b and ROS analysis of HL-60-differentiated neutrophils in vitro. Three independent experiments were collectively presented. CD11c knock out was conducted by CRISPR-Cas9 with 2 different guide RNAs (single guide [sg]1 and sg2). For ROS, we also presented the ratio of CD11c KO-sg1 and CD11cKO-sg2 transfected cells’ ROS compared with control-sg transfected cells. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001. SEM, standard error of the mean.