Transcriptome profiling of REH B-ALL cell models after FBXW7 isoform KD or reconstitution reveals putative downstream targets. (A) Overlap of selected comparisons (α group vs β group) in RNA-Seq data from the 2 REH cell models. Blue: KD model gene list, differentially expressed genes in MO α vs MO β. Red: Reconstitution model gene list, differentially expressed genes in α reconstitution (KO+a) vs β reconstitution (KO+b). Each group includes replicates from 2 independent experiments. (B) List of overlapping downstream targets that exhibit opposite directionality of transcript changes in the 2 models. (C) Taqman qPCR of the putative downstream target genes in the KD (top) and reconstitution (bottom) models. Arrows indicate directionality of transcript changes comparing the α and β groups. Data represent means ± SEM of 2 independent experiments. One-way ANOVAs of all groups were performed followed by Tukey comparisons between the α and β groups. ∗Adjusted P < .05, ∗∗adjusted P < .01, ∗∗∗adjusted P < .001.