Generation of viable gain-of-function REH B-ALL cell models in which FBXW7 isoforms were knocked out and reconstituted. (A) Experimental approach for stable reconstitution of FBXW7 isoforms in a pan-FBXW7 KO REHCas9 single-cell clone. (B) Taqman qPCR to examine RNA expression in reconstituted REHCas9 FBXW7 KO cells. Relative expression was normalized to REH parental cells. (C-D) Lack of effects on cell cycle progression of FBXW7 isoform reconstitution in KO cells. (E-F) Lack of effects on cell viability of FBXW7 isoform reconstitution in KO cells. Data representative of 2 independent experiments are shown in panels C and E. Data represent means ± SEM of 2 independent experiments in panels D and F. One-way ANOVA was performed, and no significant difference was found between these groups.