IGF-1 down-regulates Bim expression in the human Karpas707 cells through the PI-3K and p42/44 MAPK pathways. (C) Cells were starved 24 hours, pretreated with LY294002 (1 hour of preincubation; 10 μM) and stimulated with IGF-1 for 10 minutes. Next, the levels of p-Akt, tot-Akt, p-FoxO3a, and tot-FoxO3a were analyzed by Western blot. To confirm equal loading levels of actin were determined.