Ablation of Ulk1 reduces the beneficial effects of miR-144/451 disruption in β-thalassemia. WT C57BL/6 mice (expressing CD45.1) received transplantation with Lin⁻ E14.5 fetal liver HSPCs with the indicated genotypes and analyzed 8 weeks after transplantation. (A-D) Erythroid indices (y-axis) according to the genotype (x-axis). n = 10 to 12 mice for each genotype. (E) Representative spleens at 8 weeks after transplantation. Bars represent 0.5 cm. (F) Summary of spleen weights. n = between 8 and 11 mice for each genotype. (G) TAU gels stained with Coomassie brilliant blue showing soluble and insoluble α- and β-globin proteins in RBC lysates. (H) Relative insoluble α-globin levels in RBCs as determined by TAU gel analysis, performed as described in panel G, and quantified using automated image analysis. n = 3 to 5 mice for each genotype. (I) Flow cytometry quantification of S6 phosphorylation in maturation stage–matched bone marrow erythroblasts. n = 5 to 6 mice for each genotype. Bar charts show the data as mean values ± SD; ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.