Day 0 administration results in transcriptional profiles consistent with more activated CD4⁺CAR T cells and more cytotoxic CD8⁺CAR T cells compared with administration on day +5. Volcano plots show differential gene expression for day 0 compared with day +5 CAR T-cell administration for viable (A) CD4⁺ and (B) CD8⁺ CAR T cells at (left) 3, (center) 8, and (right) 16 days after CAR T-cell infusion. Combined data from 2 independent experiments are shown. FDR, false discovery rate.