Figure 5.
Expression and localization of CRISP3 in iPSC-derived neutrophil-like granulocytes and in primary cells. (A) Immunoblot analysis of lysates from iPSC-derived control and SRP-mutated iPSCs harvested at differentiation day 26. Cells were harvested at day 26, lysed, and processed for immunoblotting with the indicated antibodies. (B) Confocal images of iPSC-derived control and SRP-mutated cells stained for CRISP3. Cells were immunostained on differentiation day 26 with an anti-CRISP3 antibody and DNA was visualized with DAPI. Scale bar, 10 μm. (C) Confocal images of immunostaining of CRISP3 in a healthy donor and in a patient with the identified SRP19 gene variant. DNA was visualized with DAPI. Scale bar, 10 μm. The experiment in panels A and B was performed in triplicate, whereas the experiment in panel C twice.

Expression and localization of CRISP3 in iPSC-derived neutrophil-like granulocytes and in primary cells. (A) Immunoblot analysis of lysates from iPSC-derived control and SRP-mutated iPSCs harvested at differentiation day 26. Cells were harvested at day 26, lysed, and processed for immunoblotting with the indicated antibodies. (B) Confocal images of iPSC-derived control and SRP-mutated cells stained for CRISP3. Cells were immunostained on differentiation day 26 with an anti-CRISP3 antibody and DNA was visualized with DAPI. Scale bar, 10 μm. (C) Confocal images of immunostaining of CRISP3 in a healthy donor and in a patient with the identified SRP19 gene variant. DNA was visualized with DAPI. Scale bar, 10 μm. The experiment in panels A and B was performed in triplicate, whereas the experiment in panel C twice.

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