Tfrc^Tek-Cremice on IDD have increased LIC, express relatively low Bmp6 and Hamp mRNA levels, and induce them in response to dietary iron or holo-Tf. (A) Schematic experimental outline. All mice were 7- to 8-week-old males (n = 6-10 per group). At end point, the animals were euthanized; serum was prepared, and livers were harvested for biochemical analysis. Serum iron (B); Tf saturation (C); TIBC (D); NTBI (E); LIC (F); qPCR analysis of mRNAs encoding liver Bmp6, Bmp2, Hamp, and Id1, respectively (G-J); and mRNA/LIC ratios for Bmp6, Bmp2, Hamp, and Id1, respectively (K-N). Serum data (B-E) and LIC (F) are represented as mean ± standard error of the mean and gene expression data (G-N) are represented as geometric mean ± geometric standard deviation. Statistical differences (P < .05) were determined using Student t test on original data (B-E) or log-transformed gene expression data (G-N). Statistically significant differences from Tfrc^fl/fl and Tfrc^Tek-Cre mice on SD are represented as a or b, respectively. Dotted lines indicate the average values obtained from age-matched male Tfrc^fl/fl mice on SD (n = 6). Illustration of panel A was made with BioRender.com.