Drug screening to rescue RUNX1-deficient megakaryocyte yield. Studies of shNT- (A) and shRX-megakaryocytes (B), exposed to the indicated drugs from day 5 to day 14 of differentiation. Megakaryocyte yield was calculated by flow cytometric analysis stained for an anti-hCD42b antibody and for mCherry. The dashed line represents yield of megakaryocytes from uninfected HSPCs not exposed to any drug. Mean ± SD is shown. N = 3 to 5 separate studies, each in duplicate. ∗P ≤ .05, ∗∗∗P ≤ .001, and ∗∗∗∗P ≤ .0001 by 1-way ANOVA compared to each dimethyl sulfoxide control sample.