Figure 2.
Malignant T cells induce alterations in the epidermis. Messenger RNA (mRNA) expression was analyzed by quantitative PCR (qPCR), using β-actin as a reference gene in panels A-C. Gene expression was given as relative expression. (A) HaCaT cells were cultured for 24 hours with the nonmalignant MF1850 or malignant MF2059 T-cell line. (B) NHEK were cultured for 24 hours with supernatants obtained from the T-cell lines MF1850, MF2059, MF2000, and SeAx. (C) RHE samples were cultured in the presence of supernatants for 24 hours. (D) Protein levels were analyzed by IHC for RHE samples cultured for 48 hours with supernatants from the malignant T-cell line MF2059. The samples were stained for H&E, filaggrin, filaggrin-2, and pY-STAT3. Images are scanned by the Zeiss Axio Scan.Z1 with original magnification ×20. Scale bar; 50 μm. n = 3. ∗P < .05. KRT14, keratin protein 14; sup, supernatant.

Malignant T cells induce alterations in the epidermis. Messenger RNA (mRNA) expression was analyzed by quantitative PCR (qPCR), using β-actin as a reference gene in panels A-C. Gene expression was given as relative expression. (A) HaCaT cells were cultured for 24 hours with the nonmalignant MF1850 or malignant MF2059 T-cell line. (B) NHEK were cultured for 24 hours with supernatants obtained from the T-cell lines MF1850, MF2059, MF2000, and SeAx. (C) RHE samples were cultured in the presence of supernatants for 24 hours. (D) Protein levels were analyzed by IHC for RHE samples cultured for 48 hours with supernatants from the malignant T-cell line MF2059. The samples were stained for H&E, filaggrin, filaggrin-2, and pY-STAT3. Images are scanned by the Zeiss Axio Scan.Z1 with original magnification ×20. Scale bar; 50 μm. n = 3. ∗P < .05. KRT14, keratin protein 14; sup, supernatant.

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