MALDI-TOF spectrum of N-glycans. (A) LC-MS/MS of O-glycans released from human platelets, showing extracted ion chromatogram. The peaks were collected, and each structure was confirmed by MSⁿ (Table 3). (B) MSⁿ disassembly of isomeric fucosylated O-glycans isolated by high-performance LC (m/z 1518.9) with spectral match of Fuc(α1,2) Gal, from the standard LNFP-I (lacto-N-fucopentaose I) in MSⁿ. (C) Quantification of released O-glycans, classified by sialylation status (neutral/asialo-, monosialylated, or disialylated) was performed by extracted ion chromatographs. Example structures are depicted, with the conclusive list of structures quantified found in Table 3.