Increased platelet-leukocyte interaction in neonates. Characteristics of neonates and controls (ctrl) are displayed at t1: 0 to 2 days after birth, t2: 3 to 7 days after birth, t3: 8 to 14 days after birth. (A-B) PLA indicated as CD45+/CD41+ events in percentage of CD45+ events, (C-D) PNA indicated as CD45+/CD16+/CD41+ events in percentage CD45+/CD16+ events, (E-F) PMA indicated as CD14+/CD41+ events in percentage of CD45+, and (G) platelet-CD4+ aggregates or (H) platelet-CD8+ aggregate events were assessed by flow cytometry in whole blood. (I) Neonatal PLAs upon preincubation of whole blood with PSGL-1 blocking antibody clone KPL-1 or GPIIb/IIIa blocker eptifibatide (10 μg/ml) for 15 minutes. All graphs show median ± 95% confidence interval. Differences were analyzed using Kruskal-Wallis-test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. ns, nonsignificant.
