A refined phenotypic metric based on DNAM-1 and granzyme B levels. (A) Heatmap representing the DNAM-1 expression (MFI), using z-score normalization. C1/C1 donors (n = 37), C1/C2 donors (n = 42), and C2/C2 donors (n = 12). (B) Correlation between the percentage of educated NK cells and degranulation rates. (C) Correlation between DNAM-1 expression and granzyme B content in KIR2DL3⁺ or KIR2DL1⁺ NK cells with the degranulation rate of the same subset of NK cells. Negative correlation between KIR expression intensity and degranulation rate. (D) Unsupervised K-means clustering of the donors in the missing HLA-C1 context, based on DNAM-1 expression, granzyme B content and the size of the different educated subsets (n = 80). (E) Summary graph of the degranulation response (CD107a) in clusters from 1 to 4. (F) Unsupervised K-means clustering of the donors in the missing HLA-C2 context, based on DNAM-1 expression, granzyme B content, and the size of the different educated subsets (n = 54). (G) Summary graph of the degranulation response (CD107a) in clusters from 1 to 4. Each dot represents 1 donor. R is the Pearson coefficient. One-way ANOVA tests followed by Tukey multiple comparison tests were performed in panels D and E. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; and ∗P < .05.