MK buds are distinguishable from blebs and MVs. C57BL/6 mouse femoral cryosections were stained with antibodies against CD41 (pink), VWF (green), or phalloidin (white), and imaged using STED microscopy. (A) Representative 2D STED images showing MK buds with internal VWF, CD41, and F-actin (i), in contrast to hollow MK blebs (ii). (i, ii) Left hand panels depict merged, low magnification images, whereas the remaining panels depict a magnified bud (i) and hollow bleb; (ii) with individual fluorescence channels for VWF, CD41, and F-actin at high magnification. (B) Representative 3D STED (left panels) and 3D render (right panels) images showing VWF^– MK MVs (top panel) and VWF⁺ MK buds (lower panel). Note: The top left panel contains an inset with highlighted MVs magnified by 3.5×, and the CD41 intensity increased to illustrate MVs.