SCE5-scuPA treatment, either before or 2 hours post-photothrombotic stroke, reduces infarct volume and associated cerebral blood deposits. (A) Representative CT images of a mouse brain at 7 days post-photothrombotic stroke (without treatment). Solid arrows indicate hypointense (darker) voxels, consistent with low-density matter characteristic of infarcted cerebral tissue. Open arrows indicate hyperintense (brighter) voxels, representing higher density matter characteristic of blood deposits within the infarct. Scale bars as indicated on the image. (B and C) CT imaging–based quantification of infarct size (B) and blood volumes within the infarct (C) at 7 days poststroke in mice treated before photothrombotic stroke with saline (vehicle), SCE5-scuPA (150 U/g), or TNK-tPA (2.5 mg/kg). (D and E) Representative mean T2∗-weighted coronal and axial MRI acquisitions at 24 hours after stroke (postmortem, intra-skull) (D) and the corresponding, MRI-based quantification of infarct size (E) in mice treated 2 hours post-photothrombotic stroke with saline (D; top panels) or SCE5-scuPA (75 U/g; D; bottom panels). Efficient clearance of blood deposits (dark areas in the vehicle-treated brain; solid arrows in panel D) and reduction in the edematous (bright; open arrows in panel D) area can be observed. Scale bars = 1 mm. Data are presented as mean ± standard error of the mean. #P < .05, ####P < .0001 by one-way analysis of variance with Tukey’s or Dunnett’s post hoc analysis. ∗P < .05 by a t test (unpaired, 2-tailed). n = 4 to 11.