Kinetics of AdV-specific T cells with TCR nucleotide sequences present in the infused T-cell products in patients without viral loads after infusion. In 16 out of 17 patients that received a product that contained AdV-specific T cells, no AdV viral load was detected after infusion of the product. PB samples of these 16 patients that were obtained before and after infusion of the product were sorted by MACS for CD8⁺ T cells followed by mRNA isolation and sequencing of the TCRs. The numbers of target antigen–specific CD8⁺ T cells per liter were calculated by multiplying the frequencies of AdV-specific TCR nucleotide sequences with the absolute numbers of CD3⁺CD8⁺ T cells per liter of blood. Absolute numbers of AdV target antigen–specific T cells in PB samples are illustrated from the moment just before product infusion (day 0) until the end of follow-up. Shown are the kinetics of AdV target antigen–specific T cells in patients without detectable AdV viral loads in whom TCR nucleotide sequences that were identical to the products were found that only appeared after infusion of the products (A) and appeared before and after infusion of the products (B).