Annotation and quantification of virus-specific TCR sequences from the T-cell products. T cells from 20 T-cell products were successfully expanded. The different virus-specific T-cell populations were sorted by FACS from these expanded products into separate pure populations using pMHC tetramers followed by direct mRNA isolation and TCR sequencing of the CDR3β regions. The remaining unsorted T cells from the T-cell products were sequenced in parallel to quantify the TCRs that were present in the products. (A) The numbers of T cells from the products that were put in culture and the cell numbers after expansion are shown. The red lines represent medians. Shown are the frequencies of CMV- (B), EBV- (C), and AdV- (D) specific TCR nucleotide sequences (CDR3β sequences) that were present in the target antigen–specific T-cell products. The sum of all target antigen–specific TCR nucleotide sequences were set to 100%. The different virus specificities are shown as stacked columns for each product. The number of different CDR3β sequences are shown that were specific for CMV- (E), EBV- (F) and AdV- (G) derived antigens for each product. nt, nucleotide; ID, identification.