Figure 3.
Specification and emergence of HSCs via EHT in the human AGM region. Schematic representation of the establishment of HSC-forming HE in the human AGM region. At CS14/15, an arterially specified HOXA-patterned EC gives rise to an arterial subset termed pre-HE, characterized by ALDH1A1 (RA-signaling) and IL-33 expression. HE is specified from pre-HE by the hematopoietic transcription factor RUNX1 in cells that upregulate KCNK17, CD44, and WNT inhibitors (DKK1), and gives rise to SPINK2+CD45+ nascent HSCs. In the earlier embryo (CS10-11), HE that generates HSC-independent progenitors is specified from immature endothelial precursors that express embryonic genes (LIN28A) and lack HOXA patterning and arterial identity. (Right) Immunofluorescence validation of stage-specific markers indicate the emergence of human HSCs from IAHC in sections of CS15 human AGM. Modified from Calvanese et al.15

Specification and emergence of HSCs via EHT in the human AGM region. Schematic representation of the establishment of HSC-forming HE in the human AGM region. At CS14/15, an arterially specified HOXA-patterned EC gives rise to an arterial subset termed pre-HE, characterized by ALDH1A1 (RA-signaling) and IL-33 expression. HE is specified from pre-HE by the hematopoietic transcription factor RUNX1 in cells that upregulate KCNK17, CD44, and WNT inhibitors (DKK1), and gives rise to SPINK2+CD45+ nascent HSCs. In the earlier embryo (CS10-11), HE that generates HSC-independent progenitors is specified from immature endothelial precursors that express embryonic genes (LIN28A) and lack HOXA patterning and arterial identity. (Right) Immunofluorescence validation of stage-specific markers indicate the emergence of human HSCs from IAHC in sections of CS15 human AGM. Modified from Calvanese et al.15 

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