IL-27Rα and JAM2 are expressed on in vitro–generated PCs. (A) PCs were generated from 5 healthy donors using an established in vitro method (Cocco et al⁴³) and assessed for expression of IL-27Rα and JAM2 at various time points throughout differentiation via flow cytometry. Representative histograms are shown for IL-27Rα (dark red) and JAM2 (dark blue) compared with isotype control (light red or blue). (B) Pooled data are shown in bar charts. (C) PCs on day 13 were stimulated with rIL-27 (red), rIL-21 (red-dotted), or without (blue) for 30 minutes before intracellular staining for phosphorylated STAT1 (p-STAT1) and p-STAT3 and assessed via flow cytometry. (D) Data from 2 individual donors are shown. ∗∗P < .01; ∗∗∗P < .001 using one-way ANOVA and Tukey multiple comparison test.