Figure 5.
LILRB4 contributes to MM cell migration. (A-B) Analysis of the effect of LILRB4 on cell proliferation using the cell counting kit-8 assay. (C) Cell migration assay. HUVECs expressing green fluorescence protein were seeded on the transwell membrane (8 μm pore size). After 48 hours, LILRB4-OE and KO NCI-H929 cells expressing red fluorescence protein were cultured in the upper chamber for 24 hours. (D) Number of representative migrating cells counted using confocal microscopy. Scale bar, 50 μm. (E-F) The number of migrating LILRB4-OE and KO NCI-H929 cells. Data are means and standard error of the mean of 3 independent experiments. (G-I) Effect of LILRB4 KO and overexpression on the messenger RNA expression of angiopoietin 1, integrin αv, and integrin β3.

LILRB4 contributes to MM cell migration. (A-B) Analysis of the effect of LILRB4 on cell proliferation using the cell counting kit-8 assay. (C) Cell migration assay. HUVECs expressing green fluorescence protein were seeded on the transwell membrane (8 μm pore size). After 48 hours, LILRB4-OE and KO NCI-H929 cells expressing red fluorescence protein were cultured in the upper chamber for 24 hours. (D) Number of representative migrating cells counted using confocal microscopy. Scale bar, 50 μm. (E-F) The number of migrating LILRB4-OE and KO NCI-H929 cells. Data are means and standard error of the mean of 3 independent experiments. (G-I) Effect of LILRB4 KO and overexpression on the messenger RNA expression of angiopoietin 1, integrin αv, and integrin β3.

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