GOLM1 blocks cell apoptosis through stabilizing BCL-XL. (A) Exogenous GOLM1 associates with BCL-XL. 293T cells cotransfected with His-GOLM1 and Flag-BCL-XL, were subjected co-immunoprecipitation (IP) followed by immunoblotting (IB) as indicated antibodies. (B) Endogenous GOLM1 interacts with BCL-XL. SUP-M2 cells were subjected co-IP followed by immunoblotting (IB) as indicated antibodies. Normal immunoglobulin G (IgG) is used as control. (C) Representative images with GOLM1-GFP and BCL-XL coexpression (scale bar, 5 μm) in SUP-M2 and Karpas 299 cells. Cells were transfected with plasmid encoded GOLM1-GFP and performed immunofluerescent staining with anti- BCL-XL antibody. The colocalization of GOLM1-GFP and BCL-XL is indicated by an arrow. (D) 293T cells transfected by BCL-XL alone or with GOLM1 were treated with CHX and harvested at indicated time points. (E) The relative intensity of each band in panel D were quantitated by Image J software. (∗P < .05, ∗∗∗P < .001, 2-tailed Student t test).
